The Limits of Amylase Essay Example for Free

The Limits of Amylase EssayAbstr doingThis musical theme explains the purpose of this experiment in a way that conveys information to the reader about Amylases efficiency to withstand vitriolic or basic pH. To do this, two psychometric block out tubes were both filled with 5mL of a 5% amylase etymon. The first one was filled with an acid, while the otherwise was filled with a base. After falling liquid Iodine and benedicks beginning into separately one, the tube with a basic pH tested positive for glucose. The acerb solution tested (mostly) negative for glucose, although there were trace amounts at the bottom. Overall, my final stage is that only acidic solutions inhibit Amylases ability to digest starch.BackgroundThe goal of the experiment is to test whether or not Amylase can withstand abnormal changes in pH without denaturing itself. Salivary Amylase is an enzyme that acts upon any(prenominal) polyose that enters the mouth (mainly starch). However, it has a few o ther clinical uses. It is used for the diagnosis of acute pancreatitis, an inflammation of the pancreas, and other medical conditions regarding Amylase levels in the body.Amylases first discovery in 1831 led to new experiments about this enzyme. By separating pancreatic Amylase from Trypsin, they were able to realize that it acted upon starch, breaking it down into a simple sugar, or monosaccharide. In the plot (featured below), the active site of this enzyme containthree major acidic groups.These contain a calciumion (the double gray sphere), the chloride ion (thelarge green sphere), andthe chain of five sugarunits (in yellow orange). Previouslymentioned, this enzymewill act upon starch andbreak it down into simplesugars.2HypothesisIf we expose Amylase to a variety of acidic and basic solutions to test whether it will still digest starch or not, then I ring that it will denature both ways, because Amylase is normally intolerable to any pH above 8 or below 6.Materials and Procedu resThe following will be useful in this experiment20mL of a 5% Amylase solution20mL of a 1% starch solution8 test tubesAt least 5mL of HClAt least 5mL of NaOHHot plateBeaker full of water (for incubation)Liquid Iodine ascendantBenedicts SolutionFollow this procedure1. In your first 4 test tubes, add the following solutions accordingly to each(prenominal) tube-Test tube 1 5mL of distilled water-Test tube 2 5mL of the 5% Amylase solution-Test tube 3 5mL of Amylase solution + 2-3 drops of HCl-Test tube 4 5mL of Amylase solution + 2-3 drops of NaOH2. Add 5mL of the starch solution into each tube.3. Incubate each tube in the beaker of water for 30 minutes. The temperature should be 37C (98.6F).4. After incubation, split half(prenominal) of all contents in each tube into 4 new tubes.5. Insert 2-3 drops of the Iodine solution into the first set of beakers (the original 4). Then, insert 2-3 drops of the Benedicts solution into the second set of test tubes.6. Record any color changes.3Res ultsThe first test tube (containing distilled water) was light brown when mixed with iodine, and blue when mixed with Benedicts solution. The second test tube (5% Amylase solution) was light brown when mixed with iodine. It became orange when Benedicts was added to the mix. The third test tube turned dark brown when the iodine was added. The other beaker with test tube 3s contents remained blue when Benedicts solution was added, although traces of orangeness were found. The finis test tube appeared Light Brown with the iodine mixed in (3 drops are always used), and appeared Orange when combined with the Benedicts solution.DiscussionMany of the test tubes ended up looking kindred I expected them to, except the fourth beaker. I had not expected that Amylase, infra the influence of a very basic solution, could still act as a catalyst for starch. With a pH of9 or racyer, Amylase would normally denature itself. This could mean one of two things Amylase is capable of functioning nor mally under a very high pH, or it couldve been human error that led to this surprising result. To minimize chances of this misadventure again, assuming that this was human error only, would be to maximize the pH in the fourth beaker to 14 kinda of 12, should a new lab arise, focusing on Amylases ability to withstand a high pH without getting denatured.ConclusionOverall, most of my results agreed with my notes and background knowledge about Amylase, apart from the unlikeness in test tube 4. Like I predicted, the Amylase solution (tube 2) tested positive for simple sugars, the acidic solution denatured the enzyme, and the distilled water did absolutely nothing.SourcesDugdale, D. (2013, October 31). Amylase blood. Retrieved from http//www.nlm.nih.gov/medlineplus/ency/article/003464.htm (n.a.) Alpha- amylases. (2006, February 18). Retrieved from http//www.rcsb.org/pdb/education_discussion/molecule_of_the_month/download/Alpha-amylase.pdf (n.a.) Alpha amylase. (2010, January 29). Retr ieved from http//science.marshall.edu/murraye/alpha_amylase.htm